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July 2007 WYD PPT PDF

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July 2007 WYD PPT PDF
July 2007
What’s Your Diagnosis ?
Tissue from a
mouse
H&E
2007 JH Pheno WYD 07 Brayton
1
Helicobacters in mice
Colon
• 2-3x mucosal
thickening
• Inflammation
– Chronic and
neutrophilic
• Proliferation
• Intraluminal debris +
protozoa
• Female nude mouse,
Foxn1 nu/nu
• Helicobacter +
2007 JH Pheno WYD 07 Brayton
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Entamoebae
Colon
Lumen
• Cell debris, degenerate
neutrophils and
• amoeboid trophozoites
up to 20u diam,
– some with phagocytosed
cells and debris
– Cw Entamoeba muris
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Liver
Low magnification
• Chronic and active
portal-periportal
inflammation
• Cholangiolar
proliferation
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Liver
• Chronic (primarily
lymphocytic) and active
(neutrophilic) inflammation
• Cholangiolar proliferation
• Anisocytosis, anisokaryosis,
aneuploidy,
hepatocytomegaly, and
intranuclear ‘inclusions’ of
invaginated cytoplasmic
material can be common in
older mice. A relation ship
with helicobacter or other
infections is not clear
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Liver
• Necrosis +
inflammation may
be seen in some
strains of mice (e.g.
BALB/c here) at
some stages of
infection.
(This slide is very
faded/pink).
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Liver
Warthin Starry Silver
stain
• Note that these are
smaller than
Helicobacters found in
stomachs of humans,
dogs, cats and other
species
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Liver (higher magnification than
previous image)
Modified Steiner’s Silver stain
• Helicobacters may be easier to
see with this stain
• Sensitivity of detection is low.
• Silver stains are fairly expensive
• Careful examination is time
consuming Sensitivity is low
compared to PCR
• Histologic/histochemical
examination is a terminal
procedure (for the mouse)
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Colon
Modified Steiner’s Silver
stain
• Most bacteria stain with
silver stains such as Warthin
Starry and Modified
Steiner’s
• Staining should not be
relied on to identify
Helicobacters or to
distinguish them from other
agents
2007 JH Pheno WYD 07 Brayton
9
Rectal prolapse, Proliferative colitis
= Nice IBD phenotype
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Rectal prolapse
• Perineal skin
(with Perianal
sebaceous glands)
• Colon-rectum
mucosa (with
proliferation,
inflammation,
edema)
2007 JH Pheno WYD 07 Brayton
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2007 JH Pheno WYD 07 Brayton
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Helicobacters
In mice
Often subclinical Æ Why Worry?
¾ H hepaticus Æ strain dependent dz
ª necrotizing – chronic hepatitis +ÃLiver tumors
– males more susceptible to hepatitis
ª typhlocolitis in immunodeficient + competent mice,
- females more susceptible to typhlocolitis
¾ H bilis, H hepaticus Æ chronic typhlocolitis in immunodeficient
¾ H bilis Æ human biliary Dz ?? H muridarum – chronic gastritis
¾ Rodentium, typhlonius, (Flexispira) rappini, ganmani, etc sp…
2007 JH Pheno WYD 07 Brayton
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Helicobacters in mice
Suggested References
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Clin. Microbiol., 2007. 45(7): p. 2166-2172.
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Helicobacter species." J Natl Cancer Inst 86(16): 1222-7.
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Whary, M. T., S. J. Danon, et al. (2006). "Rapid onset of ulcerative typhlocolitis in B6.129P2-IL10tm1Cgn (IL-10-/-) mice infected with Helicobacter trogontum is
associated with decreased colonization of Altered Schaedler's Flora." Infect. Immun.: IAI.01091-06.
Whary, M. T. and J. G. Fox (2004). "Natural and experimental Helicobacter infections." Comp Med 54(2): 128-58.
Zhang, L., S. J. Danon, et al. (2005). "Natural Colonization with Helicobacter species and the Development of Inflammatory Bowel Disease in Interleukin-10deficient Mice." Helicobacter 10(3): 223-230.
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